New York Medical College

Research

In-vivo Imaging System Laboratory

Department of  Pediatrics Small Animal Bioimaging Core Facility

IVIS MiceThe Department of Pediatrics Small Animal Bioimaging Core Facility is located in a 230 square foot dedicated space located in the basement of the Basic Science Building within the NYMC Animal Facility. This core provides quantitative fluorescence and bioluminescence imaging services for the in-vivo and in-vitro study of small animals and specimens. The tracking of cell populations in vivo, as in animal models, is essential for sophisticated studies, especially stem cell and cancer biology research. The fate and differentiation of cells derived from transplanted stem cells, for instance, can be non-invasively followed in-vivo using bioluminescent or fluorescent tags monitored by a camera. IVIS SystemThe facility’s dedicated imaging instrument is the Xenogen In-vivo Imaging System (IVIS) SPECTRUM ™  from Caliper Life Sciences which is a in-vivo biophotonic imaging technology to facilitate exploration of gene expression, cell trafficking,  pathogens and tumors or disease progression in living animals. It possesses multimodal imaging which provides both quantitative bioluminescence and fluorescence imaging for up to 4 mice or 2 medium rats. It can also accommodate petri dishes or microtiter plates for in-vitro imaging.  This system includes a highly sensitive CCD camera, light-tight imaging chamber and complete automation and analysis capabilities. It also includes premium handling equipment such as a heated stage, gas anesthesia connections and ECG monitoring. High resolution, 21 sharp cut-off filters are interchangeable to achieve the highest performance, sensitivity and spectral un-mixing in fluorescence and bioluminescence imaging. In addition, the instrument performs 3D diffuse tomography to determine the geometry and depth or intensity of fluorescence.IVIS Mice in Color It includes Living Image 3.1 software to yield reproducible quantitative results. Applications are diverse and are not limited to: oncology research, infectious disease, inflammation, metabolic disease, neurology, gene therapy, cardiovascular disease, immunology and transplantation biology, toxicology and drug metabolism studies.

Service Request:
Investigators who would like to request services from the facility should download the appropriate request form for the use of the IVIS instrument and email the completed form back to mitchell_cairo@nymc.edu and janet_ayello@nymc.edu. The PI and Department or Division/Fiscal Officer  of the investigator program will be required to sign the request form. IVIS Petri PlateThese core services are provided based on the order  of the signed service form received. Once the animal protocol approval and NYMC account approval is verified, you will be notified by email for assigned date(s) and time(s). The fee in 2011/2012 will initially be set at $100./hour for use in this Core Facility.

Contact:

Mitchell S. Cairo, MD, Core Director
Chief, Pediatric Hematology, Oncology & Stem Cell Transplantation
Director, Children's & Adolescent Cancer and Blood Disease Center
Medical and Scientific Director, Cell and Tissue Engineering Laboratory
Associate Chairman, Department of Pediatrics
Professor of Pediatrics, Medicine, Pathology, Microbiology & Immunology and Cell Biology & Anatomy
Maria Fareri Children's Hospital at Westchester Medical Center
New York Medical College
Munger , Room 110
Tel: 914/ 594-3650
FAX: 914/594-3803

Email: mitchell_cairo@nymc.edu

Janet Ayello, MS, MT(ASCP), Core Lab Manager
Manager, Pediatric Cancer Research Laboratory
Manager, Cell and Tissue Engineering Laboratory
Tel: 914/594-3728 or 914/493-1671
Fax: 914/ 594-3779

Email: janet_ayello@nymc.edu

Page updated: November 28, 2011