NYMC > Research > Core Facilities & Shared Resources > Core Histology Laboratory

Histopathology Laboratory and Florescence Imaging Core

The Histopathology Laboratory and Florescence Imaging Core is located in the Department of Pathology in the Basic Science Building, room 421. The core provides routine histology, special staining, immunohistochemistry, immunofluorescence, and cryotomy services to research community of NYMC as well as outside entities and institutions. The core has full time personnel responsible for day-to-day operations and training of users on Imaging Core Instrumentation.

The Histology Laboratory accepts fixed wet tissue specimens (in labeled tissue cassettes) as well as frozen tissue samples. The core can provide paraffin and frozen sections. Sections can be prepared on standard or charged slides and stained with either Hematoxylin and Eosin (H&E) or special stains, or left unstained. The lab is semi-automated to provide consistent results and quick turnaround. Our histology protocols strive to maintain the highest possible specimen quality and minimize the possibility of cross contamination.

Equipment:

Leica Microdissection system LMD 6500 is used for obtaining well-defined starting material for subsequent downstream applications such as genomics, RNA transcript profiling, proteomics and microarrays. With this instrument one can quickly locate a single cell or large group of cells, technically draw and cut and remove specific cells for subsequent molecular analysis. Regardless of whether one uses paraffin-embedded or frozen tissue samples, stained or immuno-labeled slides, the LMD preserves the exact morphologies of both the captured cells as well as the surrounding tissue. This microscope has the following objectives:

  • 40x HCX Plan FL 0.6na CORR XT
  • 20x  HCX Plan FL 0.4na CORR
  • 10x HC Plan FL 0.3na
  • 5x UVI Microdissection 0.12na

Nikon 90i Eclipse Research microscope is fully automated for light and fluorescent microscopy. Cubes available are DAPI, GFP (488) and Texas Red (593). Software is available for various morphometric analysis. Objectives available at:

  • 4X Plan Fluor
  • 10X Plan Fluor
  • 20X Plan Fluor40X Plan Apo
  • 60X oil Plan Apo
  • 100X oil Plan Apo

Zeiss LSM980 plus Airyscan 2 Confocal System is a high-resolution high sensitivity advanced confocal imaging system that is fully equipped for 3D and 4D imaging of live and fixed cells and tissues as well as photobleaching studies. It is located in the Basic Science Building room 245. The system microscope objectives include:

  • Super-resolution 63x1.4 NA high resolution objective
  • 10x/0.3 NA
  • 20x/0.8 NA air objective
  • Long Distance 40X/1.2 NA Multi-Immersion objective

Other equipment: Leica ASP300 tissue processor, Leica CM1850 cryostat, Sakura Tissue-Tek TEC-5 tissue embedding console system.

Core Pricing:

Service

Unit

 Current NYMC Price

 

 

For Inside Services 

For Outside Services

Process and embed

1 Block

$8.00

$16.00

Decal, process and embed

1 Block

$12.00

$24.00

OCT/TFM embedding

1 Block

$12.00

$24.00

Frozen sectioning

1 Slide

$2.00

$4.00

Unstained slide (paraffin or frozen sections)

     regular

     charged

1 Slide

1 Slide

$2.00

$2.50

$4.00

$5.00

H&E stain (paraffin or frozen sections)

1 Slide

$3.00

$6.00

 

 

Special Stains

 

Masson Trichrome, Periodic Acid Schiff (PAS), Elastin stains

Setup

$50.00

$100.00

1 Slide

$5.00

$10.00

 

 

Immunohistochemistry

 

One antibody, counterstain with hematoxylin

Setup

$50.00

$100.00

1 Slide

$10.00

$20.00

Double labeling with antibodies, counterstain with hematoxylin

Setup

$50.00

$100.00

1 Slide

$12.00

$24.00

Antibody optimization

per antibody

$250.00

$500.00

 

 

 

Pathology consultations

 

 

 

Projects consultation or evaluation of slides

per hour

price varies

 

 

 

Equipment Usage

 

Nikon 90i Eclipse research microscope

per hour

$20.00

$40.00

Nikon Eclipse TI-E inverted microscope system

per hour

$20.00

$40.00

Leica Laser Capture

per hour

$30.00

$60.00

Service Request:

Investigators who would like to request services from the laboratory should fill out a requisition and the PI is required to sign the request form. The services provided will be based on the order it is received. 

Contact:

For histology services:

Tetyana Cheairs, M.D., M.S.P.H.
Core Director
Tel. (914) 594-3105
Email: Tetyana_Kobets@nymc.edu

S M Shafiqul Alam
Core Lab Manager
Tel. (914) 594-4862
Email: salam8@nymc.edu

For pathology consultations:

Humayun Islam, M.D., Ph.D.
Chair, Department of Pathology, Microbiology and Immunology
Tel. (914) 594-4150
Fax: (914) 594-4163
Email: Humayun.Islam@wmchealth.org