The Lipidomics and Mass Spectrometry Core is located in the Department of Pharmacology and provides support for faculty and student researchers both here at New York Medical College and at other institutions and private companies. Our staff provides a wide range of expertise and will work with you during the planning and protocol development stages to ensure excellent results. We can also provide figures to assist in manuscript preparation.
The core’s primary analytical instrument is a Shimadzu LC-MS/MS 8050 system, which is equipped with a UHPLC and auto-sampler. The LC-MS/MS allows for the identification and quantification of lipid mediators from biological matrices as it is both highly selective and sensitive without the need for time consuming derivatization. The sensitivity differs between analytes and depends on the sample matrix; we generally can detect 0.1 to 1 pg of standards on column.
We can detect both free and esterified fatty acids (after a hydrolysis step). Our current list of lipid mediators include the arachidonic acid (AA)-derived eicosanoids, docosahexaenoic acid (DHA) and eicosapentaenoic (EPA)-derived resolvins, protectins, and maresins also collectively called specialized pro-resolving mediators (SPMs). See full list here (link to table below). We are constantly updating this list and can design specialized custom methods for almost any fatty acid.
All users please acknowledge the NYMC Lipidomics & Mass Spectrometry Core in their publications.
The LC-MS/MS service is available as a fee-for-service. The cost per sample depends on the sample work-up needed and how many analytes you wish to quantify. Please e-mail us for current pricing.
Brendon Naicker, Core Manager
(914) 594-4939 • bnaicker@nymc.edu
Michal L. Schwartzman, Ph.D.
Professor and Chair of the Department of Pharmacology
michal_schwartzman@nymc.edu
Compounds |
[M-H] |
|
Cyclooxygenase Products |
|
|
PG F2a |
333 |
|
8-iso PG F2a |
353 |
|
PG D2 |
351 |
|
PG E2 |
351 |
|
Lipoxygenase Eicosanoids |
|
|
LXA4 |
351 |
|
LXB4 |
351 |
|
5,15-diHETE |
335 |
|
5,12-diHETE |
335 |
|
12-OxoETE |
335 |
|
5-OxoETE |
317 |
|
12-HETrE |
321 |
|
15-HETrE |
321 |
|
15-HETE |
319 |
|
12-HETE |
319 |
|
5-HETE |
319 |
|
Specialized Pro-resolving Mediators (SPMs) |
|
|
Protectin D1 |
359 |
|
Maresin 1 |
359 |
|
Maresin 2 |
359 |
|
Resolvin D5 |
359 |
|
Resolvin D1 |
375 |
|
Resolvin D2 |
375 |
|
Resolvin D3 |
375 |
|
17-HDHA |
343 |
|
14-HDHA |
343 |
|
13-HDHA |
343 |
|
7-HDHA |
343 |
|
4-HDHA |
343 |
|
|
|
|
CYP450 Eicosanoids |
|
|
14,15-DiHETrE |
337 |
|
11,12-DiHETrE |
337 |
|
8,9-DiHETrE |
337 |
|
5,6-DiHETrE |
337 |
|
14(15)-EET |
319 |
|
11(12)-EET |
319 |
|
8(9)-EET |
319 |
|
5(6)-EET |
319 |
|
20-HETE |
319 |
|
19-HETE |
319 |
|
18-HETE |
319 |
|
|
External |
Internal |
|
1-8 analytes Free Fatty Acids Esterified Fatty Acids (requires hydrolysis) |
$125/sample $145/sample |
$85/sample $105/sample |
|
>8 analytes Free Fatty Acids Esterified Fatty Acids (requires hydrolysis) |
$165/sample $185/sample |
$105/sample $125/sample |
Addition of internal standards, lipid extraction, data acquisition and complete data analysis in the form of tables and graphs.
