Lipidomics and Mass Spectrometry Core
The Lipidomics and Mass Spectrometry Core is located in the Department of Pharmacology and provides support for faculty and student researchers both here at New York Medical College and at other institutions and private companies. Our staff provides a wide range of expertise and will work with you during the planning and protocol development stages to ensure excellent results. We can also provide figures to assist in manuscript preparation.
The LC-MS/MS allows for quantification and identification of lipid mediators from biological matrices as it is both highly selective and sensitive without the need for time consuming derivation. Our newest ultra sensitive instrument, a Shimadzu LC MS-8050, is equipped with a UPLC and auto-sampler. Other instruments include an AB Sciex 3200 Qtrap system which is equipped with an Agilent HPLC and auto-sampler (3200-Qtrap); this model is sensitive enough for most applications. We are able to run a large amount of samples overnight because of our gas generators from PEAK Scientific Parker Balston. Sensitivity differs between analytes and depends on sample matrix; however we generally can detect 0.1 to 1 pg of standards on column.
We can detect both free and esterified fatty acids (after a hydrolysis step). Our current list of lipid mediators include:
- the arachidonic acid (AA)-derived eicosanoids
- docosahexaenoic acid (DHA)
- eicosapentaenoic (EPA)-derived resolvins, protectins, and maresins also collectively called specialized pro-resolving mediators (SPMs)
You can see the full list of compounds below. We are constantly updating this list and can design specialized customs methods for almost any fatty acid.
The GC/MS system (Agilent 6890N/5973) has been set up for carbon monoxide (CO) analyses in samples from in vitro and in vivo studies.
We ask that all users please acknowledge the NYMC Lipidomics & Mass Spectrometry Core in your publications.
Compounds
| Cyclooxygenase Products | [M-H] |
|---|---|
| PG F2a | 333 |
| 8-iso PG F2a | 353 |
| PG D2 | 351 |
| PG E2 | 351 |
| Lipoxygenase Eicosanoids | [M-H] |
|---|---|
| LXA4 | 351 |
| LXB4 | 351 |
| 5,15-diHETE | 335 |
| 5,12-diHETE | 335 |
| 12-OxoETE | 335 |
| 5-OxoETE | 317 |
| 12-HETrE | 321 |
| 15-HETrE | 321 |
| 15-HETE | 319 |
| 12-HETE | 319 |
| 5-HETE | 319 |
| Specialized Pro-resolving Mediators (SPMs) | [M-H] |
|---|---|
| Protectin D1 | 359 |
| Maresin 1 | 359 |
| Maresin 2 | 359 |
| Resolvin D5 | 359 |
| Resolvin D1 | 375 |
| Resolvin D2 | 375 |
| Resolvin D3 | 375 |
| 17-HDHA | 343 |
| 14-HDHA | 343 |
| 13-HDHA | 343 |
| 7-HDHA | 343 |
| 4-HDHA | 343 |
| CYP450 Eicosanoids | [M-H] |
|---|---|
| 14,15-DiHETrE | 337 |
| 11,12-DiHETrE | 337 |
| 8,9-DiHETrE | 337 |
| 5,6-DiHETrE | 337 |
| 14(15)-EET | 319 |
| 11(12)-EET | 319 |
| 8(9)-EET | 319 |
| 5(6)-EET | 319 |
| 20-HETE | 319 |
| 19-HETE | 319 |
| 18-HETE | 319 |
Fees
Both the LC-MS/MS and GC/MS services are available as a fee for service. The cost per sample depends on sample work-up needed and how many analytes you wish to quantify. Please e-mail us for exact estimate.
| Service Type | Internal | External |
|---|---|---|
| 1-8 analytes | $125/sample | $85/sample |
| Esterified (requires hydrolysis) | $145/sample | $105/sample |
| >8 analytes | $165/sample | $105/sample |
| Esterified (requires hydrolysis) | $185/sample | $125/sample |
Addition of internal standards, lipid extraction, data acquisition and complete data analysis in the form of tables and graphs.
Contacts
Brendon Naicker, Core Manager
Basic Sciences Building Room 509D
Phone: (914) 594-4939
Email: bnaicker@nymc.edu
Michal L. Schwartzman, Ph.D.
Professor and Chair of the Department of Pharmacology
Email: michal_schwartzman@nymc.edu
